化学
马来酸
共聚物
膜
木筏
两亲性
形状记忆合金*
脂筏
苯乙烯
膜蛋白
生物化学
生物物理学
肉豆蔻酰化
脂质微区
高分子化学
有机化学
生物
聚合物
数学
组合数学
作者
Karel Harant,Tomáš Čajka,Pavla Angelisová,Jana Pokorná,Václav Hor̆ejšı́
标识
DOI:10.1016/j.bpc.2023.106989
摘要
An advantageous alternative to the use of detergents in biochemical studies on membrane proteins are the recently developed styrene-maleic acid (SMA) amphipathic copolymers. In our recent study [1] we demonstrated that using this approach, most T cell membrane proteins were fully solubilized (presumably in small nanodiscs), while two types of raft proteins, GPI-anchored proteins and Src family kinases, were mostly present in much larger (>250 nm) membrane fragments markedly enriched in typical raft lipids, cholesterol and lipids containing saturated fatty acid residues. In the present study we demonstrate that disintegration of membranes of several other cell types by means of SMA copolymer follows a similar pattern and we provide a detailed proteomic and lipidomic characterization of these SMA-resistant membrane fragments (SRMs).
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