Multi-color fluorescence sensing platform for visual determination of norfloxacin based on a terbium (Ш) functionalized covalent organic framework

Sensitive and visual determination of fluoroquinolone antibiotics (FQs) is of great significance since their abuse and inappropriate handling can be problematic. Herein, we propose a lanthanide covalent organic framework fluorescence sensing system (Tb@COF-Ru) with visualization capability to determine the FQs level, where Tb@COF was employed as the sensing probe, while the red-emitting Ru(bpy)32+ serves as a constant red fluorescent background. With increasing norfloxacin concentration, the green fluorescence of Tb3+ is gradually enhanced, finally realizing the multicolor fluorescence change from red to green. With a smartphone for RGB analysis, visual monitoring and quantitative analysis were realized without any sophisticated instrument. Limits of detection for the fluorescence quantitative and visual mode for norfloxacin were 0.33 nM and 7.3 μM, respectively. This method was rapid (1 min) and visualized, providing a simple analysis of various food matrices (honey, milk, egg and beef) and water samples for trace FQs.