糖基化
表位
抗体
细胞生物学
肽序列
细胞外
跨细胞
计算生物学
细胞
生物
生物化学
免疫学
基因
内吞作用
作者
Friedrich‐Christian Deuschle,A. Schiefner,Arne Skerra
出处
期刊:Proteins
[Wiley]
日期:2019-04-23
卷期号:87 (8): 693-698
被引量:8
摘要
Abstract The CD98 heavy chain (CD98hc) constitutes both a promising cell surface target for the treatment of cancers and a transcytosis receptor potentially useful for the brain delivery of therapeutics. However, pharmacokinetic studies and safety assessment of cognate antibodies or nonimmunoglobulin binding proteins in rodents is hampered by cross‐species variability of both amino acid sequence and glycosylation pattern. Here, we report the crystal structure of the murine CD98hc extracellular domain and a comprehensive comparison with its human ortholog, revealing only one conserved surface patch that is neither shielded by glycosylation nor by the cell membrane with an accessible surface area typical for an antibody epitope. Our results imply the necessity of a surrogate approach for CD98hc‐specific binding proteins with predictive power for clinical investigations.
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