吸光度
校准曲线
藻类
分光光度法
光密度
环境化学
化学
色谱法
植物
物理
生物
检出限
光学
作者
LÃocia Helena Ribeiro Rodrigues,Alexandre Arenzon,Maria Teresa Monica Raya-Rodriguez,Nelson Ferreira Fontoura
出处
期刊:Journal of Environmental Chemistry and Ecotoxicology
[Academic Journals]
日期:2011-08-31
卷期号:3 (8): 225-228
被引量:54
摘要
The unicellular algae Pseudokirchneriella subcapitata (Korschikov) Hindák has been frequently used for ecotoxicological tests.In this paper, a calibration curve is proposed correlating absorbance values (684 nm) and cell density for routine ecotoxicological experiments.Density (cells/mL) could be estimated as follow: Cell Density = e {[ln (absorbance_684) +16.439]/1.0219}(n=130; r 2 =0.9998).Residual distribution revealed that the equation could be applied for algal densities under 5,000,000 cells/mL and/or absorbance values as high as 0.5.
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