MXene have been recognized as a highly versatile material because of its high metallic conductivity, excellent ion-transmission properties, good biocompatibility, large surface area and ease of functionalization, which make it as a promising candidate for the development novel biosensors. Since the introduction of the two-dimensional martials for probing neural activities, increasing numbers of researches were performed to functionalize enzymes or biomaterials on the surface of MXene for biosensing applications. In this mini-review, we summarize and discuss the latest advances in MXene-based materials for biomedical applications.