莲花
水解物
抗氧化剂
化学
DPPH
保健品
水解
大小排阻色谱法
食品科学
分子质量
EC50型
莲花效应
色谱法
生物化学
植物
生物
酶
体外
有机化学
原材料
作者
Yi‐Ping Yu,Shu-Jung Lai,Chien-Ru Chang,Wen‐Chi Chen,Shih‐Hsiung Wu,Chun‐Ping Lu
标识
DOI:10.1016/j.lwt.2021.111138
摘要
Lotus (Nelumbo nucifera Gaertn.) seeds are rich in proteins and are considered to be a beneficial source of plant proteins. In this study, lotus seed protein hydrolysate (LSPH) was prepared using Flavourzyme and antioxidant activities were evaluated. The LSPH possessed the highest DPPH-scavenging (EC50 2.9 mg/mL), H2O2-scavenging (EC50 16.1 mg/mL), and reducing power activity (8.0 mg/mL) at 180 min of hydrolysis. After gel filtration and reverse phase HPLC purification, the low molecular weight fraction was then analyzed by Nano-LC-MS/MS. Sixteen peptides with corresponding mass from 1 to 2 kDa were identified. Peptides with hydrophobic features and acidic amino acids, Asp and Glu, are considered to contribute to overall antioxidant activities. These results show Flavourzyme hydrolysis of lotus seed protein successfully generates low molecular fragments that make them a potential nutraceutical or health promoting supplement.
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