生物分析
寡核苷酸
分析物
色谱法
化学
吸附
检出限
纳米技术
材料科学
有机化学
DNA
生物化学
作者
Jennifer M. Nguyen,Martin Gilár,Brooke M. Koshel,Michael Donegan,Jason MacLean,Zhimin Li,Matthew Lauber
出处
期刊:Bioanalysis
[Newlands Press Ltd]
日期:2021-08-01
卷期号:13 (16): 1233-1244
被引量:12
标识
DOI:10.4155/bio-2021-0115
摘要
Aim: Accurate and reliable quantification of oligonucleotides can be difficult, which has led to an increased focus on bioanalytical methods for more robust analyses. Recent advances toward mitigating sample losses on liquid chromatography (LC) systems have produced recovery advantages for oligonucleotide separations. Results & methodology: LC instruments and columns constructed from MP35N metal alloy and stainless steel columns were compared against LC hardware modified with hybrid inorganic-organic silica surfaces. Designed to minimize metal-analyte adsorption, these surfaces demonstrated a 73% increase in 25-mer phosphorothioate oligonucleotide recovery using ion-pairing reversed-phase LC versus standard LC surfaces, most particularly upon initial use. Conclusion: Hybrid silica chromatographic surfaces improve the performance, detection limits and reproducibility of oligonucleotide bioanalytical assays.
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