分子信标
寡核苷酸
荧光
体内
癌细胞
纳米颗粒
细胞内
体外
核糖核酸
化学
胶体金
生物物理学
癌症
癌症研究
生物
纳米技术
分子生物学
DNA
材料科学
生物化学
基因
遗传学
物理
量子力学
作者
Zhuo Yang,En Liu,Su Min Wang,Yu Xiao,Shuo Zeng,Shi Chun Yang,Xianda Zhao,Yu Huang
出处
期刊:Nanomedicine
日期:2021-10-01
卷期号:16 (25): 2255-2267
被引量:1
标识
DOI:10.2217/nnm-2021-0249
摘要
Aim: Long noncoding RNA (lncRNA) BC032469-dependent gold nanoparticle molecular beacons (AuNP-MB) were constructed for the detection of gastric cancer cells. Materials & methods: The AuNP-MBs were prepared according to well-established procedures based on the Au–S interaction between the gold lattice and thiol functionalized oligonucleotides. More importantly, the stability and targeting ability of AuNP-MB were verified by a series of in vitro and in vivo experiments. Results: The lncRNA-dependent probes were successfully utilized for AuNP-MB-based intracellular imaging, with fluorescence effectively emitted in GC cells, but not in normal cells. Notably, such fluorescent emission was positively correlated with lncRNA BC032469 expression. Conclusion: The authors developed an effective fluorescent imaging probe for the recognition of gastric cancer cells.
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