适体
化学
严重急性呼吸综合征冠状病毒2型(SARS-CoV-2)
2019年冠状病毒病(COVID-19)
试剂
穗蛋白
2019-20冠状病毒爆发
纳米技术
组合化学
生物物理学
色谱法
病毒学
分子生物学
爆发
传染病(医学专业)
物理化学
病理
材料科学
疾病
生物
医学
作者
Liu Shi,Lin Wang,Xuemei Ma,Xiaona Fang,Liangliang Xiang,Yongxiang Yi,Jinlong Li,Luo Z,Genxi Li
出处
期刊:Analytical Chemistry
[American Chemical Society]
日期:2021-11-30
卷期号:93 (49): 16646-16654
被引量:38
标识
DOI:10.1021/acs.analchem.1c04156
摘要
With the outbreak of COVID-19, which is fast transmitting and highly contagious, the development of rapid, highly specific, and sensitive detection kits has become a research hotspot. The existing assay methods for SARS-CoV-2 are mainly based on enzymatic reactions, which require expensive reagents, hindering popular use, especially in resource-constrained areas. Herein, we propose an aptamer-based method for the assay of SARS-CoV-2 via binding of the spike protein using functionalized biomimetic nanochannels. To get the analogous effect of human ACE2, a receptor for the spike protein, the aptamer to bind to the spike S1 protein has been first screened by a SELEX technique and then immobilized on the previously prepared nanochannels. In the presence of SARS-CoV-2, the changes in steric hindrance and charge density on the surface of the nanochannels will affect the ion transport, along with a rapid electrochemical response. Our method has been successfully applied to detect the viral particles in clinical pharyngeal swab specimens in one step without sample treatment. We expect this rapid, reagent-free, and sensitive assay method to be developed as a useful tool for diagnosing COVID-19.
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