Chemical synthesis of peptides characterisation of cdnas encoding neuropeptide precursors in_situ hybridization in the vertebrate nervous system strategies for the ultrastructural study of peptide-containing neurones separation and determination of neuropeptides using hplc with electrochemical detection release of neuropeptides in_vitro neuropeptide release in_vivo - measurement in hypophysial portal blood neuropeptide-degrading enzymes characterisation of neuropeptide receptors using photoaffinity and biotinylated analogs second messenger responses to neuropeptides.