细胞器
荧光显微镜
流式细胞术
共焦显微镜
染色
共焦
显微镜
细胞生物学
细胞仪
显微镜
电子显微镜
人口
数字图像分析
化学
生物
荧光
病理
分子生物学
计算机科学
光学
医学
物理
环境卫生
遗传学
计算机视觉
标识
DOI:10.1002/0471142956.cy0904s14
摘要
Functional analysis of cellular organelles can be accomplished by staining cells with suitable organelle-specific dyes and analyzing the fluorescence of the stained cells with a flow cytometer. With this methodology it is possible to resolve suspected heterogeneity in organelle function or content within a population of cells. Morphological information can be provided by quantitative microscopy (confocal microscope or video microscope with digital image-analysis system). The thirteen protocols cover flow cytometry and microscopy for both live and fixed cells.
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