Establishment of Liver Cancer Model from CBRH-7919 Rat Liver Cancer Cell Line in Immune-Suppressed Rats Monitored by Ultrasound

Objective To monitor the establishment of liver cancer model in immune-suppressed rats with ultrasound.MethodsThe immune system of one rat was inhibited by 60 Co irradiation and intraperitoneal injection of Hydrocortisone,and the rat received a subcutaneous inoculation of CBRH-7919 rat liver cancer cells.After 2 weeks' growth,the tumor was transplanted into left liver of other 20 immune-suppressed rats.Then the sonographic and color Doppler features of the tumors were observed by ultrasound.About 2 weeks after the transplantation,the rats were killed and liver tumors were examined pathologically.Results Three out of the 20 rats died during the experiment.About 2 weeks after tumor transplantation,ultrasonography demonstrated that liver tumors grew in 17 rats,and the average diameter of liver tumors was (1.02± 0.15 )cm,thus the tumor-take rate was 85%.The pathological examination showed that the tumors were hepatocellular carcinoma.ConclusionA rat liver cancer model has been established by immune-suppression and monitored by ultrasound,and may used as an ideal animal model for diagnosis and local therapy of liver cancer.