Skin Tissue Engineering,Fabrication and Biocompatibility Evaluation of Cell Scaffold

The development of skin tissue engineering provides a non invasive method for skin restoration.As one of three key factors in tissue engineering,the cell scaffolds play an important role.To meet the requirements of cell scaffolds for tissue engineering in respect of mechanical property,physical structure and biocompatibility,the porous scaffolds of poly(DL lactide)(PDLLA),and poly(lactide co caprolactone)(PLACL)were first fabricated,then they were implanted into the muscle of rat back.The rats died at different times after implantation and the retrieved implants from each rat were observed and compared with acellular dermis matrix (ADM)having good biocompatibility.It was found that the degradation rate,mechanical properties,porosity,and pore size of PDLLA and PLACL scaffolds can be adjusted according to the requirements of skin tissue engineering.There were no obvious inflammatory cells after implanting of the materials,and the formed vasa in the scaffolds became similar with normal vasa and distributed evenly after 21 days.The biocompatibility of PDLLA and PLACL is not as good as ADM,but the foreign body reactions were not obvious.The scaffolds of PDLLA and PLACL can meet elementary requirements of skin tissue engineering.This study provides meaningful and experimental basis for further study of artificial skin of PLACL.