颜料
色谱法
表征(材料科学)
高效液相色谱法
萃取(化学)
化学
类胡萝卜素
吡啶
材料科学
有机化学
纳米技术
生物化学
作者
J. L. Garrido,Sylvie Roy
出处
期刊:Methods in molecular biology
日期:2015-01-01
卷期号:: 241-252
被引量:4
标识
DOI:10.1007/978-1-4939-2684-8_15
摘要
HPLC is still the technique of choice for the analysis and characterization of phytoplankton pigments. In this chapter we describe procedures for sample preparation and pigment extraction, and the use of octyl silica columns and pyridine-containing mobile phases to separate chlorophylls and carotenoids. The identification of pigments on the basis of their retention times and visible spectra, the preparation of pigment standards, and the quantitative analysis by either external or internal standard procedures are also described.
科研通智能强力驱动
Strongly Powered by AbleSci AI