保质期
潮湿
血红蛋白
红细胞
化学
DNA损伤
离体
体内
细胞生物学
生物物理学
生物化学
体外
DNA
生物
食品科学
生物技术
物理
气象学
作者
Subhashini Pandey,Manohar Mahato,Preethem Srinath,Utkarsh Bhutani,Tanu Jain Goap,Priusha Ravipati,Praveen Kumar Vemula
标识
DOI:10.1038/s41467-022-35269-3
摘要
Abstract Transfusion of healthy red blood cells (RBCs) is a lifesaving process. However, upon storing RBCs, a wide range of damage-associate molecular patterns (DAMPs), such as cell-free DNA, nucleosomes, free-hemoglobin, and poly-unsaturated-fatty-acids are generated. DAMPs can further damage RBCs; thus, the quality of stored RBCs declines during the storage and limits their shelf-life. Since these DAMPs consist of either positive or negative charged species, we developed taurine and acridine containing electrospun-nanofibrous-sheets ( Tau-Acr NFS), featuring anionic, cationic charges and an DNA intercalating group on their surfaces. We show that Tau-Acr NFS are efficient in scavenging DAMPs from stored human and mice RBCs ex vivo. We find that intermittent scavenging of DAMPs by Tau - Acr NFS during the storage reduces the loss of RBC membrane integrity and reduces discocytes- to -spheroechinocytes transformation in stored-old-RBCs. We perform RBC-transfusion studies in mice to reveal that intermittent removal of DAMPs enhances the quality of stored-old-RBCs equivalent to freshly collected RBCs, and increases their shelf-life by ~22%. Such prophylactic technology may lead to the development of novel blood bags or medical device, and may therefore impact healthcare by reducing transfusion-related adverse effects.
科研通智能强力驱动
Strongly Powered by AbleSci AI