生物
转录组
基因
核糖核酸
信使核糖核酸
RNA序列
RNA甲基化
表观遗传学
胚胎发生
小桶
基因表达
遗传学
甲基化DNA免疫沉淀
抄写(语言学)
细胞生物学
甲基化
分子生物学
甲基转移酶
DNA甲基化
语言学
哲学
作者
Yaqing Chang,Yushan Lai,Runzhou Liu,Lin Ye,Xiao‐Qiang Yu,Xiaoyun Wang
出处
期刊:Insect Science
[Wiley]
日期:2023-02-23
卷期号:30 (5): 1229-1244
被引量:1
标识
DOI:10.1111/1744-7917.13172
摘要
N6 -methyladenosine (m6 A) RNA is the most abundant modification of mRNA, and has been demonstrated in regulating various post-transcriptional processes. Many studies have shown that m6 A methylation plays key roles in sex determination, neuronal functions, and embryonic development in Drosophila and mammals. Here, we analyzed transcriptome-wide profile of m6 A modification in the embryonic development of the destructive agricultural pest Spodoptera frugiperda. We found that the 2 key mRNA m6 A methyltransferases SfrMETTL3 and SfrMETTL14 have high homologies with other insects and mammals, suggesting that SfrMETTL3 and SfrMETTL14 may have conserved function among different species. From methylated RNA immunoprecipitation sequencing analysis, we obtained 46 869 m6 A peaks representing 8 587 transcripts in the 2-h embryos after oviposition, and 41 389 m6 A peaks representing 9 230 transcripts in the 24-h embryos. In addition, 5 995 m6 A peaks were differentially expressed including 3 752 upregulated and 2243 downregulated peaks. Functional analysis with Gene Ontology and Kyoto Encyclopedia of Genes and Genomes suggested that differentially expressed m6 A peak-modified genes were enriched in cell and organ development between the 2- and 24-h embryos. By conjoint analysis of methylated RNA immunoprecipitation-seq and RNA-seq data, we found that RNA m6 A methylation may regulate the transcriptional levels of genes related to tissue and organ development from 2- to 24-h embryos. Our study reveals the role of RNA m6 A epigenetic regulation in the embryonic development of S. frugiperda, and provides new insights for the embryonic development of insects.
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