阿拉伯糖
多糖
甘露糖
拉伤
单糖
半乳糖
三七
16S核糖体RNA
发酵
根际
化学
生物
生物化学
细菌
基因
遗传学
替代医学
病理
木糖
解剖
医学
作者
Inhyup Kim,Geeta Chhetri,Yoonseop So,Sunho Park,Yonghee Jung,Hyeonseok Woo,Taegun Seo
出处
期刊:Microorganisms
[MDPI AG]
日期:2023-07-27
卷期号:11 (8): 1900-1900
被引量:2
标识
DOI:10.3390/microorganisms11081900
摘要
Microbial exopolysaccharides (EPSs) have attracted attention from several fields due to their high industrial applicability. In the present study, rhizosphere strain CJ11T was isolated from the root of Glycine max L. in Goyang-si, Republic of Korea, and a novel exopolysaccharide was purified from the Lysobacter sp. CJ11T fermentation broth. The exopolysaccharide’s average molecular weight was 0.93 × 105 Da. Its monosaccharide composition included 72.2% mannose, 17.2% glucose, 7.8% galactose, and 2.8% arabinose. Fourier-transform infrared spectroscopy identified the exopolysaccharide carbohydrate polymer functional groups, and the structural properties were investigated using nuclear magnetic resonance. In addition, a microstructure of lyophilized EPS was determined by scanning electron microscopy. Using thermogravimetric analysis, the degradation of the exopolysaccharide produced by strain CJ11T was determined to be 210 °C. The exopolysaccharide at a concentration of 4 mg/mL exhibited 2,2-diphenyl-1-picrylhydrazyl free-radical-scavenging activity of 73.47%. Phylogenetic analysis based on the 16S rRNA gene sequencing results revealed that strain CJ11T was a novel isolate for which the name Lysobacter soyae sp. nov is proposed.
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