Extraction of super high-yield lignin-carbohydrate complexes from rice straw without compromising cellulose hydrolysis

Lignin-carbohydrate complexes (LCC) that exhibit both structural advantages of lignin and carbohydrates are promising amphiphilic biopolymers, but the extraction is challenged by its liable chemical bond cleavage between lignin and carbohydrates. This work proposed a facile chemical route to integrating the production of water-insoluble (WIS LCC) and water-soluble LCC (WS LCC) into the emerging deep eutectic solvent (DES) biorefinery at mild conditions. The tailored mechanochemical fractionation process of ball milling assisted aqueous alkaline DES could extract 24.2 % LCC in total, with the co-production of a highly hydrolysable cellulose fraction (98.7 % glucose conversion). The resulting LCC exhibited considerably high contents of β-O-4, phenyl glycoside, and ferulic acid linkage bonds. When 100 g starting straw was subjected to this technique route, 9.1 g WIS LCC, 15.1 g WS LCC and 45.5 g glucose were cascaded produced. It was proposed that the selective disruption of hydrogen bonding entangled network and the quasi-state dissolution of the whole biomass allowed the subsequent cascade fractionation of WIS LCC, WS LCC and highly hydrolysable cellulose through solution property adjustment. This work showed a promising approach for LCC production with high yield without compromising cellulose conversion potential, which has been challenging in the current lignocellulose biorefinery.