毛螺菌科
厚壁菌
肠道菌群
蛋白质细菌
生物
乳酸菌
关节炎
类风湿性关节炎
代谢物
点头
发病机制
免疫学
失调
内分泌学
细菌
糖尿病
遗传学
16S核糖体RNA
作者
Yeye Ma,Wenjing Li,Sijia Niu,Xiaoying Zhu,Maolin Chu,Weiyan Wang,Weihao Sun,Xuemin Wei,Juan Zhang,Zhiyi Zhang
标识
DOI:10.1016/j.intimp.2023.110885
摘要
Recent studies suggested that altered gut microbiota may be related to the pathogenesis of rheumatoid arthritis (RA), albeit the exact mechanisms are unknown. In this study, we aimed to discover the particular mechanism of RA treatment by microbiota by investigating the effects of ferroptosis on gut microbiota and its metabolites in collagen-induced arthritis (CIA) mice. Mice were divided into five groups: control, CIA, erastin, BzATP, and BzATP + erastin group. We performed 16S rDNA sequencing and metabolomics analysis on mouse feces and found that erastin and BzATP altered the microbiota and metabolites. The findings demonstrated that the microbiota was significantly disturbed at the phylum (Proteobacteria, Firmicutes, and Bacteroidota) and genus level (Lachnospiraceae_NK4A136, Lactobacillus, and Bifidobacterium) in the CIA group, and erastin exacerbated this disturbance. Unexpectedly, BzATP treatment could repair the disruptive effects of erastin. Additionally, there were significant variations in metabolites between each group. Erastin worsened metabolite abnormalities in CIA mice, while BzATP mitigated them, consistent with the microbiota results. These findings provide novel perspectives and insights into the therapy of RA.
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