检出限
分子印迹聚合物
介电谱
循环伏安法
材料科学
微分脉冲伏安法
线性范围
分析物
电极
电化学气体传感器
安培法
纳米技术
生物医学工程
化学
电化学
色谱法
医学
生物化学
选择性
物理化学
催化作用
作者
Benachir Bouchikhi,Alassane Diouf,Moulay Mustapha Ennaji,Nezha El Bari
出处
期刊:Elsevier eBooks
[Elsevier]
日期:2023-01-01
卷期号:: 317-347
标识
DOI:10.1016/b978-0-12-824156-1.00016-9
摘要
The development of noninvasive and enzyme-free devices is nowadays very popular in medical research due to their affordable cost and also to bypass the restrictions because of the measurements requiring blood. For this purpose, this work presents the development of noninvasive electrochemical sensors operating in secreted human physiological fluids, namely, urine and saliva. The proposed electrochemical devices are based on molecularly imprinted polymer (MIP) for the detection of two analytes (creatinine and glucose) that are very important in understanding kidney diseases. The challenges facing research in this field are to bypass classical techniques which are either cumbersome, expensive, or invasive. Indeed, biomimetic receptors are designed on screen-printed gold electrodes using two different ways (sandwich and electropolymerization). Here, electrochemical and morphological characterizations were performed. On the one hand, cyclic voltammetry, differential pulse voltammetry, and electrochemical impedance spectroscopy have allowed to visualize the electrochemical behavior of the proposed MIP sensors. On the other hand, scanning electron microscopy coupled with energy-dispersive spectroscopy and atomic force microscopy have allowed to study the surface properties, thereby obtaining very promising metrological properties. In a wide linear range of 0.1–1 μg/mL, a low detection limit of 0.016 ng/mL was reached for the creatinine sensor. For glucose detection, an Limit Of Detection (LOD) of 0.59 μg/mL was obtained in the working range from 0.5 to 50 μg/mL. Using partial least squares regression, the two MIP sensors were satisfactorily applied for the practical detection of both analytes in urine and saliva while referring to the Jaffé method and a glucometer, respectively.
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