视蛋白
生物
感光色素
基因组
RNA干扰
遗传学
转录组
基因
视紫红质
基因沉默
基因复制
进化生物学
基因表达
植物
核糖核酸
视网膜
作者
Jiafeng Lü,Ze‐Dong Li,Zhuang-Xin Ye,Hai‐Jian Huang,Jianping Chen,Junmin Li,Chuan‐Xi Zhang
出处
期刊:BMC Genomics
[Springer Nature]
日期:2023-06-26
卷期号:24 (1)
被引量:2
标识
DOI:10.1186/s12864-023-09470-7
摘要
Abstract Background As one of the components of visual photopigments in photoreceptor cells, opsin exhibits different spectral peaks and plays crucial roles in visual function. Besides, it is discovered to evolve other functions despite color vision. However, research on its unconventional function is limited nowadays. With the increase in genome database numbers, various numbers and types of opsins have been identified in insects due to gene duplications or losses. The Nilaparvata lugens (Hemiptera) is a rice pest known for its long-distance migration capability. In this study, opsins were identified in N. lugens and characterized by genome and transcriptome analyses. Meanwhile, RNA interference (RNAi) was carried out to investigate the functions of opsins, and then the Illumina Novaseq 6000 platform-based transcriptome sequencing was performed to reveal gene expression patterns. Results Four opsins belonging to G protein-coupled receptors were identified in the N. lugens genome, including one long-sensitive opsin ( Nllw ) together with two ultraviolet-sensitive opsins ( NlUV1/2 ) and an additional new opsin with hypothesized UV peak sensitivity ( NlUV3-like ). A tandem array of NlUV1/2 on the chromosome suggested the presence of a gene duplication event, with similar exons distribution. Moreover, as revealed by spatiotemporal expression, the four opsins were highly expressed in eyes with age-different expression levels. Besides, RNAi targeting each of the four opsins did not significantly affect the survival of N. lugens in phytotron, but the silencing of Nllw resulted in the melanization of body color. Further transcriptome analysis revealed that silencing of Nllw resulted in up-regulation of a tyrosine hydroxylase gene ( NlTH ) and down-regulation of an arylalkylamine-N-acetyltransferases gene ( NlaaNAT ) in N. lugens , demonstrating that Nllw is involved in body color plastic development via the tyrosine-mediated melanism pathway. Conclusions This study provides the first evidence in a Hemipteran insect that an opsin ( Nllw ) takes part in the regulation of cuticle melanization, confirming a cross-talk between the gene pathways underlying the visual system and the morphological differentiation in insects.
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