A Pump-Free Optofluidic Biosensing Platform Based on Whispering Gallery Mode Microspheres

Optofluidic biosensors offer significant advantages for lab-on-a-chip chemical and biological analysis by combining microfluidics for small-volume liquid manipulation with the high sensitivity and adaptability of optical sensing techniques. In this study, we present an optofluidic biosensing platform utilising a micropillar array coupled with active whispering gallery mode (WGM) microsphere resonators for the rapid detection of influenza viruses. The WGM microspheres are coated with polyclonal antibodies capable of targeting both intact virus and fragments of viral proteins. The micropillar array facilitates continuous sampling and measurement through evaporation-driven fluid transport.