Efficacy Assessment of Sulfated Flavanol Functionalized Silver Nanoparticles against MCF-7 Breast Cancer

Aim: The present research work aims to formulate a cost-effective and less toxic drug for specific and selective delivery at the tumor site. Background: Existing therapeutics, such as chemo, surgery, etc., for fast-paced MCF-7 breast cancer, still face challenges, especially due to their toxic side effects. The unique properties of metal nanoparticles embedded in anti-oxidant plant polymers have sparked great interest in the formulation of less toxic-targeted drugs for cancer cure. Objective: The present work aims to synthesize a targeted formulation of colloidal nanosilver by surface engineering of silver nanoparticles using plant polymers in electrolytic deposition technique, developed indigenously at the institute lab. Methods: A current is passed through an elctrolyte, AgNO3, which splits it into ions. The positive ions of Ag deposit over LDPE wrapped carbon cathode and negative ion, NO3, is liberated. Ag+ ions get capped in-situ. These surface-modified silver nanoparticles formulate a colloidal solution. UV-visible and FTIR spectroscopy, TEM-EDX, and XRD were used to validate asprepared formulation and in vivo human tumor xenograft model in NOD-SCID mouse for efficacy against MCF-7 breast cancer. Results: The as-synthesized formulation consists of pure spherical poly-dispersed silver nanoparticles of average size 5.4 nm, coated with sulphated flavanols. The efficacy evaluation reported that it significantly, T/C = 0.53, reduced tumor volumes with a 100% survival rate and change in animal body weight <4 gms. Conclusion: The as-synthesized formulation can be used as a potential neo-adjuvant or adjuvant drug along with existing therapeutics for MCF-7 breast cancer, significantly reducing the toxicity and cost.