Subcellular Metabolic Tracking Using Fluorescent Nanodiamonds Relaxometry

Abstract Fluorescent nanodiamonds (FNDs) relaxometry holds promising future for advancement of high spatiotemporal resolution metabolic imaging technology. In this study, a simultaneous integration of spatial position tracking with FND relaxometry is demonstrated to characterize the temporal dynamics of metabolic processes, thereby enhancing the capability to monitor cellular activities over time. To enable targeted metabolic probing in living cells, FNDs are surface‐functionalized to achieve specific localization within key organelles, including the nucleus and mitochondria. This strategy not only facilitates subcellular‐level metabolic monitoring but also allows for direct comparison between intra‐ and extranuclear microenvironments within the same living cell, offering substantial potential for elucidating the spatial and functional heterogeneity of cellular metabolism.