Engineering Nanoclusters of Cell Adhesive Ligands on Biomaterial Surfaces: Superior Cell Proliferation and Myotube Formation for Skeletal Muscle Tissue Regeneration

Abstract Engineering biointerfaces with nanoscale clustering of integrin‐binding cell adhesive peptides is critical for promoting receptor redistribution into signaling complexes. Skeletal muscle cells are exquisitely sensitive to integrin‐mediated signaling, yet biomaterials supporting myogenesis through control of the density and nanodistribution of ligands have not been developed. Here, materials are developed with tailorable cell adhesive ligands distribution at the interface by independently controlling their global and local density to enhance myogenesis, by promoting myoblast growth and myotube formation. To this end, RGD‐functionalized low‐fouling polymer surfaces with global ligand densities (G) from 0–7 µg peptide/mg polymer and average local ligand densities (L) from 1–6.3 ligands/cluster, are generated and characterized. Cell studies demonstrate improvements in cell adhesion, spreading, growth, and myotube formation up to a density of 7 µg peptide/mg polymer with 4 ligands/cluster. Optimizing ligand density and distribution also promotes early myofiber maturation, identified by increased MF20 marker protein expression and sarcomere‐forming myotubes. At higher ligand densities, these cell properties are decreased, indicating that ligand multivalency is a critical parameter for tailoring cell‐material interactions, to a certain threshold. The findings provide new insights for designing next‐generation biomaterials and hold promise for improved engineering of skeletal muscle.