神经科学
钾通道
脆性X综合征
脆性x
生物
医学
遗传学
生物物理学
基因
作者
Danijela Bataveljić,Helena Pivoňková,Vidian de Concini,Betty Hébert,Pascal Ezan,Sylvain Briault,Alexis-Pierre Bemelmans,Jacques Pichon,Arnaud Menuet,Nathalie Rouach
标识
DOI:10.1038/s41467-024-47681-y
摘要
Abstract Fragile X syndrome (FXS) is an inherited form of intellectual disability caused by the loss of the mRNA-binding fragile X mental retardation protein (FMRP). FXS is characterized by neuronal hyperexcitability and behavioral defects, however the mechanisms underlying these critical dysfunctions remain unclear. Here, using male Fmr1 knockout mouse model of FXS, we identify abnormal extracellular potassium homeostasis, along with impaired potassium channel Kir4.1 expression and function in astrocytes. Further, we reveal that Kir4.1 mRNA is a binding target of FMRP. Finally, we show that the deficit in astroglial Kir4.1 underlies neuronal hyperexcitability and several behavioral defects in Fmr1 knockout mice. Viral delivery of Kir4.1 channels specifically to hippocampal astrocytes from Fmr1 knockout mice indeed rescues normal astrocyte potassium uptake, neuronal excitability, and cognitive and social performance. Our findings uncover an important role for astrocyte dysfunction in the pathophysiology of FXS, and identify Kir4.1 channel as a potential therapeutic target for FXS.
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