蛋氨酸
吖啶橙
化学
生物化学
串联
超分子化学
组合化学
氨基酸
有机化学
材料科学
晶体结构
复合材料
细胞凋亡
作者
Zhe Zheng,Siying Ren,Wen‐Chao Geng,Xuexian Cui,Biao Wu,Hong Wang
标识
DOI:10.1002/asia.202200106
摘要
Methionine is an essential amino acid involved in many physiological and pathological processes. Methionine starvation caused by methionine decarboxylase (MetDC) degradation becomes a promising strategy for cancer treatment. Multistep colorimetric method, the present approach to monitor the MetDC activity, possesses drawbacks of the complicated process, low accuracy, and poor anti-interference due to indirect detection. Herein, we report a facile and easy-to-use supramolecular tandem assay (STA) with the cucurbit[7]uril and acridine orange reporter pair for the direct and real-time monitoring of MetDC activity. This strategy not only provides a feasible method for enzymatic activity detection but also establishes the capability of inhibitor screening.
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