A Liquid Interfacial SERS Platform on a Nanoparticle Array Stabilized by Rigid Probes for the Quantification of Norepinephrine in Rat Brain Microdialysates

Abstract For the reliable determination of trace chemicals in the brain, we created a SERS platform based on a functionalized AuNPs array formed at a liquid/liquid interface in a uniform fashion over a large substrate area through ternary regulations for real‐time quantification of trace norepinephrine (NE). The rigid molecule, 4‐(thiophen‐3‐ylethynyl)‐benzaldehyde (RP1) was designed and co‐assembled at AuNPs with 4‐mercaptophenylboronic acid (MPBA) to chemically define NE via dual recognition. Meanwhile, the rigid structure assembly of RP1 and MPBA efficiently fixed the interparticle gap, guaranteeing reproducible SERS analysis. Furthermore, the Raman peak of C≡C group in the silent region was taken as a response element to further improve the accuracy. Combined with microdialysis, this SERS platform was developed for in‐the‐field testing of NE in rat brain microdialysates following anxiety.