粘蛋白
回肠
分泌物
粘蛋白2
污渍
空肠
微生物学
免疫荧光
化学
肠道菌群
生物
免疫学
生物化学
抗体
基因表达
基因
作者
Geng Wang,Haidong Wang,Yuyue Jin,Xiao Zhang,Muhammad Umar Yaqoob,Yueying Lin,Haohan Chen,Minqi Wang
标识
DOI:10.1016/j.foodres.2022.111003
摘要
Present study was conducted to comprehensively investigate the protective effects of galactooligosaccharides (GOS, 100%) against LPS-induced intestinal barrier damages, and the regulatory effect for intestinal microbes. Results showed that GOS intervention restored villi (jejunum and ileum) integrity, which were atrophic and broken in LPS-challenged mice. Electron microscopy, western blotting and immunofluorescence analysis exhibited that mice administrated with GOS showed higher expression of tight junction, which was confirmed in IPEC-J2 cells model. Meanwhile, the GOS increased the secretion of mucin and SIgA, as well as it alleviated inflammatory response caused by LPS in NF-κB dependent way. Administration of GOS could also increase the relative abundances of several specific friendly bacteria, and enhance the adaptability of intestinal microbiota. Collectively, these results indicated the potential of GOS for protecting intestine from injuries caused by stress as LPS challenge.
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