γ-氨基丁酸受体
苦毒毒素
化学
生物物理学
乙醇
荧光
小脑
受体
生物化学
内分泌学
生物
量子力学
物理
作者
A. Christine Engblom,I. Holopainen,Karl E.O. Åkerman
出处
期刊:Brain Research
[Elsevier]
日期:1991-12-24
卷期号:568 (1-2): 55-60
被引量:20
标识
DOI:10.1016/0006-8993(91)91378-e
摘要
Cl- fluxes through the GABAA receptor gated ion channels in cultured rat cerebellar granule cells were measured using the chloride-sensitive fluorescent probe SPQ (6-methoxy-N-(3-sulphopropyl)quinolinium) incorporated into the cells. The fluorescence of SPQ is quenched by Cl- ions. The cells were bathed in a low Cl- medium so that the Cl- gradient was directed outward. Ethanol increased the SPQ fluorescence indicating a decrease in intracellular Cl- due to Cl- efflux. Picrotoxin inhibited the effect at low concentrations of ethanol (less than 50 mM) in a concentration dependent manner. The effects of ethanol were potentiated at low concentrations (less than 10 microM) of gamma-aminobutyric acid (GABA), but inhibited at higher concentrations (0.3-2.0 mM). The results support the hypothesis that ethanol may act via the GABAA receptor gated ion channel. The results also suggest that SPQ is a suitable probe for measuring GABAA receptor-coupled Cl- fluxes through the GABAA receptor-gated channels in living cells.
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