后代
海马结构
生物
程序性细胞死亡
神经元
生物发生
体视学
男科
线粒体生物发生
胎儿
辐照
线粒体
怀孕
内分泌学
细胞凋亡
内科学
神经科学
遗传学
医学
基因
物理
核物理学
作者
Hubert Korr,H. Rohde,Jens Benders,Manuel Dafotakis,Norman Grolms,Christoph Schmitz
标识
DOI:10.1080/09553000010028467
摘要
Purpose : Apart from subsequent cell death, little is known about long-term effects of a prenatal low-dose X-irradiation (PLDI) on nuclear (n) and mitochondrial (mt) DNA, and whether these effects are connected with reduced neuron numbers in the adult brain. Materials and methods : Pregnant mice were X-irradiated with 0, 10 or 50cGy at day 13 (E13) of pregnancy. One day after (E14), or postnatally at day 25 (P25) or P180, the brains of the offspring were analysed concerning the extent of nDNA repair, mt biogenesis, and the relative content of nDNA single strand breaks (SSB). Stereology was applied for evaluating neuronal loss. Results : One day after irradiation no unrepaired SSB were detected. Significant results were mainly obtained for hippocampal pyramidal cells at P180, particularly cell loss following 50 cGy PLDI, increased SSB content and mt biogenesis (0 vs. 10cGy) but decreased mt biogenesis for 10 vs. 50 cGy. Conclusions : A hypothesis closely related to that regarding molecular events during aging is presented for explaining this second wave of cell death in adult mice following PLDI as a result of accumulated mtDNA damage caused by PLDI. A possible relation to the neurodegenerative hypothesis of schizophrenia is discussed.
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