溶菌酶
毛细管电泳
色谱法
化学
质谱法
毛细管电泳-质谱法
甲酸
毛细管作用
分析化学(期刊)
串联质谱法
电喷雾电离
生物化学
材料科学
复合材料
作者
Monika Kondeková,Vítězslav Maier,Pavlína Ginterová,Jozef Marák,Juraj Ševčı́k
标识
DOI:10.1016/j.foodchem.2013.12.078
摘要
Some methodological aspects of an on-line combination of capillary zone electrophoresis with mass spectrometric detection (CZE–QqQ–MS) were studied in this work as well as the possibilities of using this combination for analysis of the high-molecular mass compounds present in multi-component matrices. All experiments using an on-line combination of capillary electrophoresis with mass spectrometric detection were carried out in cationic mode in covalently-coated capillary. The optimised electrolyte system consisted of 100 mmol/L formic acid. Prior to the CZE–QqQ–MS analysis, an extraction of lysozyme from cheese samples using 1 mol/L of acetic acid was performed. The LOD was 3.6 mg lysozyme per kg and the LOQ was 10.9 mg lysozyme per kg. The concentration range of the lysozyme determined in four cheese samples analysed in this work was from 0.5 to 3.3 g of lysozyme per kg. The values of the relative standard deviations thus obtained were from 4.6% to 9.3% depending on the cheese sample.
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