This study was conducted to investigated the effects of stocking density on growth and survival of sea cucumber, Apostichopus japonicus in the indoor multiple tank for 12 weeks. There were six treatments(stocking densities) in this experiment, i.e. 1, 2, 3, 4, 5 and 10 individuals per 10 L plastic aquarium(W41×H24×D11 ㎝, represented as D1, D2, D3, D4, D5 and D10, respectively). Each treatment had ten replicates. The results showed growth variation of sea cucumber, Apostichopus japonicus decreased with the increase of stocking densities. After the end of the experiment, no significant differences(P>0.05) between D1~D5 treatment group whereas significant differences(P<0.05) between D1 and D10 treatment group. Survival rate showed D1~D10 were 90%, 90%, 100%, 95%, 94% and 72%, respectively. Especially, significant differences(P>0.05) between D1~D5 and D10 treatment group. The results indicated optimum of stocking density for sea cucumber, Apostichopus japonicus within five individuals per 10 L plastic aquarium(0.05 individuals/㎡).