夹紧
膜片钳
脂肪细胞
脂肪组织
离子通道
原代培养
间质细胞
细胞生物学
生物
化学
解剖
内科学
内分泌学
细胞培养
医学
计算机科学
电生理学
受体
夹紧
遗传学
计算机视觉
作者
Yanhui Zhang,Dan Tong,Anil Mishra,Litao Xie,Isaac Samuel,Jessica Smith,Rajan Sah
出处
期刊:Methods in molecular biology
日期:2017-01-01
卷期号:: 145-150
被引量:2
标识
DOI:10.1007/978-1-4939-6820-6_14
摘要
The patch-clamp technique allows for the study of ion channel activity in the native adipocyte environment to better understand the contributions of ion channels to adipocyte signaling. Here, we describe methods for isolating primary mature adipocytes from both mouse and human white adipose tissues (subcutaneous and visceral). From the same preparation, we describe how to culture and differentiate preadipocytes isolated from the stromal vascular fraction. We then describe in detail patch-clamp methods, including both whole-cell and perforated-patch configurations.
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