Effects of Cortex Lycii on the Human Umbilical Veim Cells Apoptosis Induced by H_2O_2

Objective:To explore the protection effects of Chinese herbs Cortex lycii on H2O2-induced apoptosis in the Human Umbilical Vein Cells.Methods:Make four groups of the HUVEC as normal cells group,H2O2-only deal cells group and Cortex lycii+H2O2 acting cells group.After 18～24h,flow cytometry by Annexin V/PI double stain was performed to quantify the apoptotic cells.The expressions of Bcl-2 gene was tested by RT-PCR.Results:The HUVEC was treated with Cortex lycii,the apoptosis rate decreased in different degrees.RT-PCR detection showed that H2O2 inhibit the expression of the Bcl-2 gene,and when they were treated by different concentrations of Cortex lycii,the expression of the gene increased following the different concentrations of the herbs.Conclusion:Cortex lycii could prevent H2O2-induced HUVEC cells from apoptosis in different degrees,of which the activation of the expression of Bcl-2 gene.