THE INFLUENCE OF PLANTS METABOLISM TO FLUORANTHENE AND CHRYSENE ON THE COMBINATION OF THEM WITH DNA

Metabolized by mung bean seedlings,the two kinds of polycyclic aromatic bydrocarbons,fluoranthene and chrysene,were extracted and separated from the plant.The UV spectrum and the fluo- rescence spectrum of the two kinds of chemicals had been determined before and after metabolized by plant,and the combination response of the two chemicals and their metabolites with DNA was determined by the method of fluorimetry.The result indicates that fluoranthene has no change to its fine structure of the UV and fluorescence spectrum before and after metabolized by the plant,but chrysene both change in it.In the concentration of 2.5×10 -6mol·l -1,fluoranthene and its metabolites in the sane fluorescence intense with it have no combination with DNA which presented in the concentrations of 5.0×10 -7-5.0×10 -5mol·l -1,while chrysene can do it in the same conditions.Compared with chrysene,the combination capacity of chrysene metabolites with DNA has been weakened in a little.The results indicated that fluoranthene and chrysene in parent compound have different combination capacity with DNA.Metabolized by plant to these two kinds of polycyclic aromatic hydrocarbons,which have showed different carcinogenicity in animal test,has no displayed obvious activation or inactivation action.