Study on aqueous two-phase extraction method to extract rice bran lipase

This experiment adopts the aqueous two phase extraction method to extract rice bran lipase, through determine the optimal extraction conditions of rice bran lipase,provide theoretical basis for the mass production in the future. With distilled water to the rice bran lipase crude extraction experiment, the best extraction technology was determined by the single factor experiment parameters:the ratio of material to water 1∶15,extraction temperature 40 ℃,the extraction of pH 7.0,extracting time of 1h. Aqueous two–phase extraction of crude enzyme liquid purification experiment,discusses the factors affecting the aqueous two–phase extraction,and through the orthogonal experiment to optimize the extraction conditions of PEG concentration 22.5%,20% ammonium sulfate concentration,aqueous two–phase extraction under the condition of pH 7.0,lipase purified the highest multiples.