Regulating effect of Gongqing decoction on T-cell subsets to reduce the uterine bleeding of medical abortion mice

Objectives:To explore the mechanism underlying the bleeding reducing effect of Gongqing decoction via regulating T-cell subsets.Methods:Total 40 pregnant mice were assigned into mifepristone(RU486) group(1.5 mg/(kg·d)-1),Gongqing decoction low-dose[77.04 mg/(kg·d)-1],middle-dose[154.08 mg/(kg·d)-1] and high-dose[308.16 mg/(kg·d)-1] groups randomly(n=10 for each).The volume of uterine hemorrhage was measured by the method of alkaline hematin photometric.The percentages of Th1 subset(CD3+CD4+IFNγ+),Th2 subset(CD3+CD4+IL-4+),Th17 subset(CD3+CD4+IL-17A+) and Treg subset(CD4+CD25+Foxp3+)in mice uterine tissue were detected by flow cytometry.The mRNA expressions of IFNγ、IL-4、IL-17A、TGF-β were analyzed using RT-PCR.Results:Compared to RU486 group,the uterine bleeding volume reduced significantly in Gongqing decoction groups.The percentages of Th1 and Th17 cells increased while the percentages of Th2 and Treg cells decreased after Gongqing decoction administration(P0.05).Additionally,Gongqing decoction up-regulated IFNγ and IL-17A mRNA expression and down-regulated IL-4 and TGF-β mRNA expression in a dose-depended manner(P0.05).Conclusion:Gongqing decoction reduced the uterine bleeding volume of RU486-induced abortion mice efficiently via promoting Th1 and Th17 skews.