Hydrolysis effect of alkaline protease and neutral protease by single and compound on corn protein was studied. The optimum hydrolyzing conditions for neutral protease have been determined: [E] 3544 U/g, pH 7.0, temperature 55 ℃ and time(t) 2 h; The optimum hydrolyzing conditions for alkaline protease: [E] 840 U/g, pH 8.0, temperature 55 ℃ and time 2 h. In addition, two protease compound hydrolyzing corn protein is better than the single, and its DH can achieve above 20%.