色氨酸
代谢工程
大肠杆菌
生物化学
化学
限制
生物合成
酶
生物技术
氨基酸
生物
工程类
基因
机械工程
作者
Hao Niu,Ruirui Li,Quanfeng Liang,Qingsheng Qi,Qiang Li,Pengfei Gu
出处
期刊:Journal of Industrial Microbiology & Biotechnology
[Oxford University Press]
日期:2019-01-01
卷期号:46 (1): 55-65
被引量:46
标识
DOI:10.1007/s10295-018-2106-5
摘要
L-Tryptophan is an important aromatic amino acid that is used widely in the food, chemical, and pharmaceutical industries. Compared with the traditional synthetic methods, production of L-tryptophan by microbes is environmentally friendly and has low production costs, and feed stocks are renewable. With the development of metabolic engineering, highly efficient production of L-tryptophan in Escherichia coli has been achieved by eliminating negative regulation factors, improving the intracellular level of precursors, engineering of transport systems and overexpression of rate-limiting enzymes. However, challenges remain for L-tryptophan biosynthesis to be cost-competitive. In this review, successful and applicable strategies derived from metabolic engineering for increasing L-tryptophan accumulation in E. coli are summarized. In addition, perspectives for further efficient production of L-tryptophan are discussed.
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