DNA
DNA甲基化
分子生物学
基因
胞嘧啶
甲基化
生物
发起人
生物传感器
基因组DNA
安培法
化学
计算生物学
生物化学
基因表达
电极
电化学
物理化学
作者
Eloy Povedano,Víctor Ruiz‐Valdepeñas Montiel,Aránzazu Valverde,Fernando Navarro-Villoslada,Paloma Yáñez‐Sedeño,Marı́a Pedrero,Ana Montero‐Calle,Rodrigo Barderas,Alberto Peláez‐García,Marta Mendiola,David Hardisson,Jaime Feliú,Jordi Camps,Elisabet Rodríguez-Tomàs,Jorge Joven,Meritxell Arenas,Susana Campuzano,José M. Pingarrón
出处
期刊:ACS Sensors
[American Chemical Society]
日期:2018-11-30
卷期号:4 (1): 227-234
被引量:55
标识
DOI:10.1021/acssensors.8b01339
摘要
This paper reports the preparation of versatile electrochemical biosensing platforms for the simple, rapid, and PCR-independent detection of the most frequent DNA methylation marks (5-methylcytosine, 5-mC, and/or 5-hydroxymethylcytosine, 5-hmC) both at global and gene-specific levels. The implemented strategies, relying on the smart coupling of immuno-magnetic beads (MBs), specific DNA probes and amperometric detection at screen-printed carbon electrodes (SPCEs), provided sensitive and selective determination of the target methylated DNAs in less than 90 min with a great reproducibility and demonstrated feasibility for the simultaneous detection of the same or different cytosine epimarks both at global level and in different loci of the same gene or in different genes. The bioplatforms were applied to determine global methylation events in paraffin-embedded colorectal tissues and specific methylation at promoters of tumor suppressor genes in genomic DNA extracted from cancer cells and paraffin-embedded colorectal tissues, and in serum without previous DNA extraction from cancer patients.
科研通智能强力驱动
Strongly Powered by AbleSci AI