Determination of the effects of torularhodin against alcoholic liver diseases by transcriptome analysis

Alcoholic liver disease (ALD) is a major cause of liver injury worldwide. Oxidative damage is one of the main injuries caused by ALD. The aim of this study was to elucidate the preventive effects of torularhodin, extracted from Sporidiobolus pararoseus, on alcoholic liver injury in mice. The mechanisms involved were investigated using transcriptome analysis. Torularhodin supplementation decreased ethanol-induced aspartate transaminase (ALT), aspartate transaminase (AST) and low density lipoprotein (LDL) levels, and increased high density lipoprotein (HDL) levels in the serum of mice. In liver tissue, treatment with torularhodin increased ethanol-induced superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) levels and decreased tumor necrosis factor-α (TNF-α) and interleukin-1β (IL-1β) levels. Histological analysis showed that torularhodin could alleviate the negative effects of alcohol on the liver. Transcriptomic analysis showed that 806 genes were significantly differentially expressed (506 up-regulated and 300 down-regulated) after torularhodin treatment. These genes were involved in three main Gene Ontology categories (biological process, cellular component, and molecular function) and multiple pathways. Therefore, torularhodin was considered to have potential as a protective agent against ALD.