反硝化细菌
生物
氧化亚氮还原酶
酸杆菌
反硝化
温室气体
亚硝酸盐还原酶
生态系统
生态学
环境化学
硝酸盐
硝酸还原酶
细菌
氮气
16S核糖体RNA
放线菌门
化学
有机化学
遗传学
作者
Sergio E. Morales,Theodore F. Cosart,William E. Holben
出处
期刊:The ISME Journal
[Springer Nature]
日期:2010-02-25
卷期号:4 (6): 799-808
被引量:266
摘要
Nitrogen fixing and denitrifying bacteria, respectively, control bulk inputs and outputs of nitrogen in soils, thereby mediating nitrogen-based greenhouse gas emissions in an ecosystem. Molecular techniques were used to evaluate the relative abundances of nitrogen fixing, denitrifying and two numerically dominant ribotypes (based on the > or =97% sequence similarity at the 16S rRNA gene) of bacteria in plots representing 10 agricultural and other land-use practices at the Kellogg biological station long-term ecological research site. Quantification of nitrogen-related functional genes (nitrite reductase, nirS; nitrous oxide reductase, nosZ; and nitrogenase, nifH) as well as two dominant 16S ribotypes (belonging to the phyla Acidobacteria, Thermomicrobia) allowed us to evaluate the hypothesis that microbial community differences are linked to greenhouse gas emissions under different land management practices. Our results suggest that the successional stages of the ecosystem are strongly linked to bacterial functional group abundance, and that the legacy of agricultural practices can be sustained over decades. We also link greenhouse gas emissions with specific compositional responses in the soil bacterial community and assess the use of denitrifying gene abundances as proxies for determining nitrous oxide emissions from soils.
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