转染
细胞培养
细胞生物学
神经细胞粘附分子
细胞粘附分子
生物
互补DNA
细胞粘附
K562细胞
细胞毒性
细胞
自然杀伤细胞
分子生物学
体外
生物化学
基因
遗传学
作者
Lewis L. Lanier,Chiwen Chang,Mitsuyoshi Azuma,Joyce J. Ruitenberg,John J. Hemperly,Joseph H. Phillips
出处
期刊:Journal of Immunology
[The American Association of Immunologists]
日期:1991-06-15
卷期号:146 (12): 4421-4426
被引量:166
标识
DOI:10.4049/jimmunol.146.12.4421
摘要
Abstract The neural cell adhesion molecule (N-CAM/CD56) is a member of the Ig supergene family that has been shown to mediate homophilic binding. Several isoforms of N-CAM have been identified that are expressed preferentially in different tissues and stages of embryonic development. To examine the primary structure of N-CAM expressed in leukocytes, N-CAM cDNA were generated by polymerase chain reaction from RNA isolated from normal human NK cells and the KG1a hematopoietic leukemia cell line. The sequence of leukocyte-derived N-CAM cDNA was essentially identical with N-CAM cDNA from human neuroblastoma cells that encode the 140-kDa isoform of N-CAM. Inasmuch as N-CAM is preferentially expressed on human NK cells and a subset of T lymphocytes that mediate MHC-unrestricted cell-mediated cytotoxicity, we examined the potential role of N-CAM in cell-mediated cytotoxicity and heterotypic lymphocyte-tumor cell adhesion. N-CAM loss mutants were established from the human N-CAM+ KG1a leukemia cell line, and N-CAM cDNA was transfected into a human colon carcinoma cell line and murine L cells. Using this panel of mutants and transfectants, it was determined that expression of N-CAM on these target cells does not affect susceptibility to resting or IL-2-activated NK cell-mediated cytotoxicity. Moreover, expression of N-CAM in these transfectants failed to induce homotypic or heterotypic cellular adhesion. Collectively, these studies indicate that homophilic N-CAM interactions probably do not mediate a major role in the cytolytic interaction between NK cells and N-CAM+ tumor cell targets.
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