肝活检
内科学
医学
脂肪肝
生物标志物
纤维化
非酒精性脂肪肝
胃肠病学
血栓反应蛋白1
病理
生物
活检
疾病
生物化学
血管生成
作者
Kazuhiro Kozumi,Tatsuhiko Kodama,Hiroki Murai,Sadatsugu Sakane,Olivier Govaere,Simon Cockell,Daisuke Motooka,Naruyasu Kakita,Yukinori Yamada,Yasuteru Kondo,Yuki Tahata,Ryotaro Yamada,Hayato Hikita,Ryotaro Sakamori,Takumi Kawaguchi,Ann K. Daly,Quentin M. Anstee,Tomohide Tatsumi,Eiichi Morii,Tetsuo Takehara
出处
期刊:Hepatology
[Wiley]
日期:2021-08-21
卷期号:74 (5): 2452-2466
被引量:74
摘要
NAFLD is the most common liver disease worldwide. NASH, the progressive form of NAFLD, and advanced fibrosis are associated with poor outcomes. We searched for their noninvasive biomarkers.Global RNA sequencing of liver tissue from 98 patients with biopsy-proven NAFLD was performed. Unsupervised hierarchical clustering well distinguished NASH from nonalcoholic fatty liver (NAFL), and patients with NASH exhibited molecular abnormalities reflecting their pathological features. Transcriptomic analysis identified proteins up-regulated in NASH and/or advanced fibrosis (stage F3-F4), including matricellular glycoprotein thrombospondin-2 (TSP-2), encoded by the thrombospondin 2 (THBS2) gene. The intrahepatic THBS2 expression level showed the highest areas under the receiver operating characteristic curves (AUROCs) of 0.915 and 0.957 for diagnosing NASH and advanced fibrosis, respectively. THBS2 positively correlated with inflammation and ballooning according to NAFLD activity score, serum aspartate aminotransferase and hyaluronic acid (HA) levels, and NAFLD Fibrosis Score (NFS). THBS2 was associated with extracellular matrix and collagen biosynthesis, platelet activation, caspase-mediated cleavage of cytoskeletal proteins, and immune cell infiltration. Serum TSP-2 expression was measured in 213 patients with biopsy-proven NAFLD, was significantly higher in NASH than in NAFL, and increased parallel to fibrosis stage. The AUROCs for predicting NASH and advanced fibrosis were 0.776 and 0.856, respectively, which were comparable to Fibrosis-4 index, serum HA level, and NFS in advanced fibrosis diagnosis. Serum TSP-2 level and platelet count were independent predictors of NASH and advanced fibrosis. Serum TSP-2 levels could stratify patients with NAFLD according to the risk of hepatic complications, including liver cancer and decompensated cirrhotic events.TSP-2 may be a useful biomarker for NASH and advanced fibrosis diagnosis in patients with NAFLD.
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