大麻
四氢大麻酚
屈大麻酚
医学
色谱法
检出限
化学
药理学
麻醉
大麻素
内科学
精神科
受体
作者
Hwanjo Yu,Hoyeon Lee,Jiyong Cheong,Sang Won Woo,Juhyun Oh,Hyun-Kyung Oh,Jae-Hyun Lee,Hui Zheng,Cesar M. Castro,Yeong-Eun Yoo,Min-Gon Kim,Jinwoo Cheon,Ralph Weissleder,Hakho Lee
出处
期刊:Science Translational Medicine
[American Association for the Advancement of Science (AAAS)]
日期:2021-10-20
卷期号:13 (616)
被引量:12
标识
DOI:10.1126/scitranslmed.abe2352
摘要
Tetrahydrocannabinol (THC), the primary psychoactive ingredient of cannabis, impairs cognitive and motor function in a concentration-dependent fashion. Drug testing is commonly performed for employment and law enforcement purposes; however, available tests produce low-sensitive binary results (lateral flow assays) or have long turnaround (gas chromatography–mass spectrometry). To enable on-site THC quantification in minutes, we developed a rapid assay for oral THC analysis called EPOCH (express probe for on-site cannabis inhalation). EPOCH features distinctive sensor design such as a radial membrane and transmission optics, all contained in a compact cartridge. This integrated approach permitted assay completion within 5 min with a detection limit of 0.17 ng/ml THC, which is below the regulatory guideline (1 ng/ml). As a proof of concept for field testing, we applied EPOCH to assess oral fluid samples from cannabis users (n = 43) and controls (n = 43). EPOCH detected oral THC in all specimens from cannabis smokers (median concentration, 478 ng/ml) and THC-infused food consumers. Longitudinal monitoring showed a fast drop in THC concentrations within the first 6 hours of cannabis smoking (half-life, 1.4 hours).
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