Abstract 292: Novel Synthesis of PFC Nanobubble-magnetic Nanoparticles Conjugate for Acute Myocardial Infarction

Aims: The potential aim of this study was to prepare this conjugate and analyze its basic characteristics. This could help in myocardial salvage or reduction in infarct size which is potentially very useful. Methods and Results: Perfluorocarbon nanobubbles were prepared using standard techniques. PFC nanobubbles-magnetic nanoparticle conjugation was performed and a final solution prepared had a pH of 8.0. The conjugation was performed to form covalent bonding and PBS buffer was used. The final preparation is an emulsion with a PFC content of 25% and 40% concentration in each emulsion of 300 microl. The final volume achieved in the synthesis was 300microl of each concentration, and the final compound was stable. The final sample when evaluated under microscope gives an image shown in figure 1. The polydispersity index was <0.25. The magnetic nanoparticle concentration was 15mg/ml and the particle concentration in 300microl was the particle content was 4.5mg. A drop of emulsion subjected to the magnetic field by a small magnet tends to polarize to one side due to the effect of the magnetic field. These particles are easy and respond quickly to magnetic field. On a glass slide when allowed to dry the conjugate forms a thick film like a mountain, and when a small amount of water is added, and when a small magnet is applied nearby, the particles start moving towards the magnet. The size of the particles is about 100nm by dynamic light scattering. Conclusion: Synthesis of magnetic nanoparticle conjugation with PFC nano bubbles is feasible and stable.