Influence of nonionic surfactants on the chromatographic behaviour of proteins in hydrophobic interaction chromatography

Abstract The chromatographic characteristics of proteins in the presence of additives of nonionic surfactants Brij‐35 and Tween‐80 in the conditions of descending gradient of ammonium sulfate and phenyl‐coated polymeric stationary phase were investigated. It was revealed that retention factors of proteins may be regulated by use of mentioned additives. The improvement of resolution is achieved for some hardly separated pairs of proteins, viz. albumin egg/albumin bovine, aldolase/tripsin. A reversion of the elution order is observed for tripsin/chymotrypsinogen A. Copyright © 2003 John Wiley & Sons, Ltd.