明胶
PLGA公司
姜黄素
脚手架
移植
血管生成
化学
细胞生物学
生物医学工程
癌症研究
医学
生物化学
外科
体外
生物
作者
Pei-Chen Li,Szu-Ching Chen,Yi‐Jen Hsueh,Yang-Chun Shen,Meng‐Yu Tsai,Li-Wen Hsu,Chih‐Kuang Yeh,Hung-Chi Chen,Chieh‐Cheng Huang
标识
DOI:10.1016/j.msec.2020.111753
摘要
Corneal transplantation is currently the only approach to cure corneal blindness. Cell-based strategies that employ corneal endothelial cells (CECs) grown on supporting biomaterials hold great promise as possible alternative therapies for treating corneal endothelial dysfunction. Nevertheless, most biomaterials are used merely because of their robust mechanical properties, providing passive physical support for the transplantation of CEC monolayers. Based on the versatility of curcumin in ophthalmic applications, this study aims to develop a multifunctional scaffold system that can not only support the function and transplantation of CECs but also prevents post-engraftment complications by sustained curcumin release, thus enhancing the long-term success of CEC engraftment. Curcumin-loaded lipid-poly(lactic-co-glycolic acid) (PLGA; [email protected]) hybrid microparticles (MPs) fabricated using an oil-in-water single emulsion method are embedded into gelatin-based scaffolds. The anti-inflammatory, antioxidative, and anti-angiogenic potentials of the developed scaffolds and their capacity in supporting CEC monolayer formation are evaluated. The [email protected] are capable of promoting CEC proliferation, protecting CECs from oxidative stress-induced cell death via modulating Nrf2/HO-1 signaling axis, suppressing the secretion of pro-inflammatory cytokines by macrophages, and inhibiting the migration and angiogenesis of vascular endothelial cells. By incorporating the [email protected] into a thin gelatin membrane, the fabricated scaffold is able to support the growth and organization of CECs into a polygonal morphology with tight junctions. These experimental results demonstrate the potential of the [email protected] gelatin scaffold for actively supporting the survival and function of CEC monolayers after transplantation.
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