磁性纳米粒子
磁选
纳米颗粒
Mercury(编程语言)
离子
水溶液中的金属离子
寡核苷酸
材料科学
磁弛豫
自来水
分析化学(期刊)
化学
核磁共振
纳米技术
色谱法
磁化
磁场
DNA
有机化学
工程类
环境工程
量子力学
生物化学
物理
冶金
程序设计语言
计算机科学
作者
Yunyun Hu,Xin Guo,Hong Wang,Qiong Luo,Yang Song,Erqun Song
出处
期刊:ACS applied bio materials
[American Chemical Society]
日期:2020-03-26
卷期号:3 (5): 2651-2657
被引量:12
标识
DOI:10.1021/acsabm.0c00021
摘要
Magnetic-separation-assisted magnetic relaxation switching (MRS) assay based on the concentration change of magnetic nanoparticles switches has been designed for bacteria, biological macromolecules, and small molecules detection because of its better analysis performance. As one of the most hazardous pollutants and highly dangerous elements, mercury ion (Hg2+) was employed as a model to further investigate the applicability of nanoparticle switches concentration change-based MRS assay mode for detecting metal ions in this study. The principle is based on the specific and strong interaction between mercury ion with the thymine-thymine(T-T) mismatch in double-stranded DNA duplexes by employing oligonucleotide functionalized magnetic nanoparticle as magnetic capture probe and MRS signal probe, respectively. The result shows that magnetic nanoparticles concentration-dependent MRS sensing mode could be facile applied to detect metal ion of Hg2+ in tap water, lake water and serum with wider detection range and higher accuracy. The as-presented magnetic-separation-assisted MRS assay of Hg2+ in complicated samples shows potential application values for Hg2+ assay in clinical and environmental monitoring, which broadens its application.
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